Phytochemicals profile and in-vitro antidiabetic potentials of fractionated extracts of Entada Africana and Leptadenia Hastata

Aim: To confirm their use in the management of diabetes and to determine numerous phytochemicals present that may be connected active performance plants, fractionated extracts Leptadenia hastata Entada africana were subjected an vitro experiment. Material methods: The plant leaves dried, pulverized with a Sumeet CM/L 2128945 grinder, particle size was 45.85 ?m extracted methanol. crude using 30×8 cm diameter column 60g silica gel 60 F254 grade, methanol as eluent fractions concentrated rotary evaporator, run on thin layer Chromatographic plate (TLC) retardation factors (RF) determined. Fractions similar RF pulled together spotted again TLC final calculated. quantified for content (LH1 EA2) identified HPLC-UV detector. tested antidiabetic potentials ? -glucosidase ?-amylase enzymes in-vitro assay. Results: yields 10.0 mg (Leptadenia hastata) 11.5.0 (Entada Africana) designated LH1 EA2, EA2 0.75±0.01 0.77±0.03 respectively. maximum amount alkaloid found E. Africana (14.50±0.25 mg/g), while tannin not L. Hastata. In portion Hastata (LH1), thirteen discovered out these three alkaloids. Thirteen fraction (EA2), eight them being alkaloids flavonoids. When compared usual acarbose, plants' anti-diabetic properties superior. had EC50 0.950.17 g/ml (?-amylase) 0.970.41 (?-glucosidase), 1.00±0.11 0.90±0.35 (?-glucosidase). presence detected linked qualities leaves. Conclusion: phytochemical profile classified flavonoids are stated agents, this has proved researched plants have potential